Restart LW & then try to play the file again with the LW player. Just to make sure it's not a one-off, download another file & see whether that plays with the LW player. If things still aren't playing & you're determined to use the LW player (it's very bad), delete LW's preferences folder when LW's properly closed. See below for instructions.
http://www.gnutellaforums.com/genera...der-vista.html

If that doesn't help & you think it's a bug in the latest version, post over at this other forum. The developers don't look here but they might see your post over there.
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